diff --git a/core/io/resource_uid.cpp b/core/io/resource_uid.cpp
index 8a8c86087d4..a73adf9d747 100644
--- a/core/io/resource_uid.cpp
+++ b/core/io/resource_uid.cpp
@@ -154,6 +154,19 @@ String ResourceUID::get_id_path(ID p_id) const {
 	ERR_FAIL_COND_V_MSG(p_id == INVALID_ID, String(), "Invalid UID.");
 	MutexLock l(mutex);
 	const ResourceUID::Cache *cache = unique_ids.getptr(p_id);
+
+#if TOOLS_ENABLED
+	// On startup, the scan_for_uid_on_startup callback should be set and will
+	// execute EditorFileSystem::scan_for_uid, which scans all project files
+	// to reload the UID cache before the first scan.
+	// Note: EditorFileSystem::scan_for_uid sets scan_for_uid_on_startup to nullptr
+	//       once the first scan_for_uid is complete.
+	if (!cache && scan_for_uid_on_startup) {
+		scan_for_uid_on_startup();
+		cache = unique_ids.getptr(p_id);
+	}
+#endif
+
 	ERR_FAIL_COND_V_MSG(!cache, String(), vformat("Unrecognized UID: \"%s\".", id_to_text(p_id)));
 	const CharString &cs = cache->cs;
 	return String::utf8(cs.ptr());
diff --git a/core/io/resource_uid.h b/core/io/resource_uid.h
index 6d96953fcc1..2912168dcb4 100644
--- a/core/io/resource_uid.h
+++ b/core/io/resource_uid.h
@@ -37,6 +37,8 @@
 
 class FileAccess;
 
+typedef void (*ResourceUIDScanForUIDOnStartup)();
+
 class ResourceUID : public Object {
 	GDCLASS(ResourceUID, Object)
 public:
@@ -63,6 +65,8 @@ protected:
 	static void _bind_methods();
 
 public:
+	inline static ResourceUIDScanForUIDOnStartup scan_for_uid_on_startup = nullptr;
+
 	String id_to_text(ID p_id) const;
 	ID text_to_id(const String &p_text) const;
 
diff --git a/editor/editor_file_system.cpp b/editor/editor_file_system.cpp
index 2d10e4305ea..4c5663c3b99 100644
--- a/editor/editor_file_system.cpp
+++ b/editor/editor_file_system.cpp
@@ -48,6 +48,9 @@
 #include "scene/resources/packed_scene.h"
 
 EditorFileSystem *EditorFileSystem::singleton = nullptr;
+int EditorFileSystem::nb_files_total = 0;
+EditorFileSystem::ScannedDirectory *EditorFileSystem::first_scan_root_dir = nullptr;
+
 //the name is the version, to keep compatibility with different versions of Godot
 #define CACHE_FILE_NAME "filesystem_cache10"
 
@@ -237,16 +240,72 @@ EditorFileSystem::ScannedDirectory::~ScannedDirectory() {
 	}
 }
 
-void EditorFileSystem::_first_scan_filesystem() {
-	EditorProgress ep = EditorProgress("first_scan_filesystem", TTR("Project initialization"), 5);
+void EditorFileSystem::_load_first_scan_root_dir() {
 	Ref<DirAccess> d = DirAccess::create(DirAccess::ACCESS_RESOURCES);
 	first_scan_root_dir = memnew(ScannedDirectory);
 	first_scan_root_dir->full_path = "res://";
+
+	nb_files_total = _scan_new_dir(first_scan_root_dir, d);
+}
+
+void EditorFileSystem::scan_for_uid() {
+	// Load file structure into memory.
+	_load_first_scan_root_dir();
+
+	// Load extensions for which an .import should exists.
+	List<String> extensionsl;
+	HashSet<String> import_extensions;
+	ResourceFormatImporter::get_singleton()->get_recognized_extensions(&extensionsl);
+	for (const String &E : extensionsl) {
+		import_extensions.insert(E);
+	}
+
+	// Scan the file system to load uid.
+	_scan_for_uid_directory(first_scan_root_dir, import_extensions);
+
+	// It's done, resetting the callback method to prevent a second scan.
+	ResourceUID::scan_for_uid_on_startup = nullptr;
+}
+
+void EditorFileSystem::_scan_for_uid_directory(const ScannedDirectory *p_scan_dir, const HashSet<String> &p_import_extensions) {
+	for (ScannedDirectory *scan_sub_dir : p_scan_dir->subdirs) {
+		_scan_for_uid_directory(scan_sub_dir, p_import_extensions);
+	}
+
+	for (const String &scan_file : p_scan_dir->files) {
+		const String ext = scan_file.get_extension().to_lower();
+
+		if (ext == "uid" || ext == "import") {
+			continue;
+		}
+
+		const String path = p_scan_dir->full_path.path_join(scan_file);
+		ResourceUID::ID uid = ResourceUID::INVALID_ID;
+		if (p_import_extensions.has(ext)) {
+			if (FileAccess::exists(path + ".import")) {
+				uid = ResourceFormatImporter::get_singleton()->get_resource_uid(path);
+			}
+		} else {
+			uid = ResourceLoader::get_resource_uid(path);
+		}
+
+		if (uid != ResourceUID::INVALID_ID) {
+			if (!ResourceUID::get_singleton()->has_id(uid)) {
+				ResourceUID::get_singleton()->add_id(uid, path);
+			}
+		}
+	}
+}
+
+void EditorFileSystem::_first_scan_filesystem() {
+	EditorProgress ep = EditorProgress("first_scan_filesystem", TTR("Project initialization"), 5);
 	HashSet<String> existing_class_names;
 	HashSet<String> extensions;
 
-	ep.step(TTR("Scanning file structure..."), 0, true);
-	nb_files_total = _scan_new_dir(first_scan_root_dir, d);
+	if (!first_scan_root_dir) {
+		ep.step(TTR("Scanning file structure..."), 0, true);
+		_load_first_scan_root_dir();
+	}
 
 	// Preloading GDExtensions file extensions to prevent looping on all the resource loaders
 	// for each files in _first_scan_process_scripts.
@@ -440,6 +499,7 @@ void EditorFileSystem::_scan_filesystem() {
 		sd = first_scan_root_dir;
 		// Will be updated on scan.
 		ResourceUID::get_singleton()->clear();
+		ResourceUID::scan_for_uid_on_startup = nullptr;
 		processed_files = memnew(HashSet<String>());
 	} else {
 		Ref<DirAccess> d = DirAccess::create(DirAccess::ACCESS_RESOURCES);
diff --git a/editor/editor_file_system.h b/editor/editor_file_system.h
index 5b78b86a3f3..4f9302e045f 100644
--- a/editor/editor_file_system.h
+++ b/editor/editor_file_system.h
@@ -182,7 +182,7 @@ class EditorFileSystem : public Node {
 	static void _thread_func(void *_userdata);
 
 	EditorFileSystemDirectory *new_filesystem = nullptr;
-	ScannedDirectory *first_scan_root_dir = nullptr;
+	static ScannedDirectory *first_scan_root_dir;
 
 	bool filesystem_changed_queued = false;
 	bool scanning = false;
@@ -192,13 +192,17 @@ class EditorFileSystem : public Node {
 	float scan_total;
 	String filesystem_settings_version_for_import;
 	bool revalidate_import_files = false;
-	int nb_files_total = 0;
+	static int nb_files_total;
 
 	void _notify_filesystem_changed();
 	void _scan_filesystem();
 	void _first_scan_filesystem();
 	void _first_scan_process_scripts(const ScannedDirectory *p_scan_dir, List<String> &p_gdextension_extensions, HashSet<String> &p_existing_class_names, HashSet<String> &p_extensions);
 
+	static void _scan_for_uid_directory(const ScannedDirectory *p_scan_dir, const HashSet<String> &p_import_extensions);
+
+	static void _load_first_scan_root_dir();
+
 	HashSet<String> late_update_files;
 
 	void _save_late_updated_files();
@@ -255,7 +259,7 @@ class EditorFileSystem : public Node {
 	HashSet<String> valid_extensions;
 	HashSet<String> import_extensions;
 
-	int _scan_new_dir(ScannedDirectory *p_dir, Ref<DirAccess> &da);
+	static int _scan_new_dir(ScannedDirectory *p_dir, Ref<DirAccess> &da);
 	void _process_file_system(const ScannedDirectory *p_scan_dir, EditorFileSystemDirectory *p_dir, ScanProgress &p_progress, HashSet<String> *p_processed_files);
 
 	Thread thread_sources;
@@ -412,6 +416,8 @@ public:
 
 	static bool _should_skip_directory(const String &p_path);
 
+	static void scan_for_uid();
+
 	void add_import_format_support_query(Ref<EditorFileSystemImportFormatSupportQuery> p_query);
 	void remove_import_format_support_query(Ref<EditorFileSystemImportFormatSupportQuery> p_query);
 	EditorFileSystem();
diff --git a/main/main.cpp b/main/main.cpp
index c65e6ba8acb..6cd9c89fa8b 100644
--- a/main/main.cpp
+++ b/main/main.cpp
@@ -3444,6 +3444,17 @@ Error Main::setup2(bool p_show_boot_logo) {
 	// This loads global classes, so it must happen before custom loaders and savers are registered
 	ScriptServer::init_languages();
 
+#if TOOLS_ENABLED
+
+	// Setting up the callback to execute a scan for UIDs on disk when a UID
+	// does not exist in the UID cache on startup. This prevents invalid UID errors
+	// when opening a project without a UID cache file or with an invalid cache.
+	if (editor) {
+		ResourceUID::scan_for_uid_on_startup = EditorFileSystem::scan_for_uid;
+	}
+
+#endif
+
 	theme_db->initialize_theme();
 	audio_server->load_default_bus_layout();
 
@@ -3496,9 +3507,21 @@ void Main::setup_boot_logo() {
 
 	if (show_logo) { //boot logo!
 		const bool boot_logo_image = GLOBAL_DEF_BASIC("application/boot_splash/show_image", true);
-		const String boot_logo_path = ResourceUID::ensure_path(GLOBAL_DEF_BASIC(PropertyInfo(Variant::STRING, "application/boot_splash/image", PROPERTY_HINT_FILE, "*.png"), String())).strip_edges();
 		const bool boot_logo_scale = GLOBAL_DEF_BASIC("application/boot_splash/fullsize", true);
 		const bool boot_logo_filter = GLOBAL_DEF_BASIC("application/boot_splash/use_filter", true);
+		String boot_logo_path = GLOBAL_DEF_BASIC(PropertyInfo(Variant::STRING, "application/boot_splash/image", PROPERTY_HINT_FILE, "*.png"), String());
+
+		// If the UID cache is missing or invalid, it could be 'normal' for the UID to not exist in memory.
+		// It's too soon to scan the project files since the ResourceFormatImporter is not loaded yet,
+		// so to prevent printing errors, we will just skip the custom boot logo this time.
+		if (boot_logo_path.begins_with("uid://")) {
+			const ResourceUID::ID logo_id = ResourceUID::get_singleton()->text_to_id(boot_logo_path);
+			if (ResourceUID::get_singleton()->has_id(logo_id)) {
+				boot_logo_path = ResourceUID::get_singleton()->get_id_path(logo_id).strip_edges();
+			} else {
+				boot_logo_path = String();
+			}
+		}
 
 		Ref<Image> boot_logo;
 
@@ -4215,7 +4238,7 @@ int Main::start() {
 
 #ifdef TOOLS_ENABLED
 			if (editor) {
-				if (!recovery_mode && (game_path != String(GLOBAL_GET("application/run/main_scene")) || !editor_node->has_scenes_in_session())) {
+				if (!recovery_mode && (game_path != ResourceUID::ensure_path(String(GLOBAL_GET("application/run/main_scene"))) || !editor_node->has_scenes_in_session())) {
 					Error serr = editor_node->load_scene(local_game_path);
 					if (serr != OK) {
 						ERR_PRINT("Failed to load scene");